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Authors
Affiliations
1 Department of Microbiology, Science and Research Branch, Islamic Azad University, Fars, IR
2 Department of Microbiology, Kazerun Branch, Islamic Azad University, Kazerun, IR
Source
Nature Environment and Pollution Technology, Vol 12, No 2 (2013), Pagination: 209-214
Abstract
The purpose of this study was the evaluation of soil origin Brevibacillus for production of bioactive compounds. Nowadays bioactive compounds are considered the sources of new remedy in order to eliminate antibioticresistant bacteria. To perform the study, two strains of Brevibacillus with potent activity for production of bioactive compounds were isolated from 121 soil samples. Phenotypic and 16SrRNA sequencing identification of the isolates recognized them as Brevibacillus reuszeri (B12 bioactive compound producer) and Brevibacillus formosus (B22 bioactive compound producer). The bioactive compounds produced by the bacteria were partially purified and characterized based on their antimicrobial activities at various pHs and temperatures and proteinase K. Then, activity of the bioactive compounds was assessed against different pathogenic microorganisms. The results obtained indicated that activity of the compounds when treated with proteinase K was inhibited. The optimum pH for both the isolates was 7 and potent activity of the bioactive compound B12 was observed at 35°C while for B22 was at 40°C. Although, Bacillus cereus, Staphylococcua aureus, E. coli and Salmonella typhi were sensitive, Pseudomonas aeruginosa and fungi (Candida albicans and Aspergillus niger) were resistant to the both bioactive compounds. Therefore, Brevibacillus with potent activity for production of antimicrobial metabolites, and fast growth character may be considered as a good probiotic agent for commercial industries.
Keywords
Brevibacillus spp., 16SrRNA Sequencing, Antimicrobial Effect, Bioactive Compounds Probiotics.
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